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Incubate the membrane for 20 min and then wash with DMF to remove uncoupled amino acids. Immunofluorescent staining of Human melanoma cells, utilizing Rabbit polyclonal to acetyl Lysine (ab21623) at 1:100 dilution. Notice that the side chain has three methylene teams, so that though the terminal amino group can be charged under physiological circumstances, the side chain does have significant hydrophobic character.
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